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Figure 6: Rg3-RGE-induced signal transduction through the MAPK and its inhibitors in RAW 264.7 cells. RAW 264.7 cells were preincubated with Rg3-RGE for 30 min and then stimulated with LPS for 18 h. Nuclear and cytosolic proteins from testis and lungs were extracted using the NE-PER® Nuclear and Cytosolic Extraction Reagents. Total forms of respective factors were used as internal controls. Image is representative of three independent experiments. (a, b) Phosphorylation of MAPK pathway factors in RAW cells. (c-e) Expression levels of MAPK inhibitors. (c) PD 98059 (ERK inhibitor), (d) SB 203580(P38 inhibitor), and (e) SP 600125 (JNK inhibitor). Values in bar graphs are means ± SEM of three independent experiments. p < 0.001 are considered significant compared to the LPS-only group.