Evidence-Based Complementary and Alternative Medicine / 2018 / Article / Fig 2

Research Article

Anti-Inflammatory Potential of Carpomitra costata Ethanolic Extracts via Inhibition of NF-κB and AP-1 Activation in LPS-Stimulated RAW264.7 Macrophages

Figure 2

Effect of CCE on LPS-induced iNOS and COX-2 expression in RAW264.7 macrophages. RAW264.7 cells were pretreated with CCE (30–100 μg/mL) for 1 h and stimulated with LPS (100 ng/mL). iNOS ((a), for 24 h) and COX-2 ((b), for 7 h) protein levels were analyzed in whole cell lysates using western blotting. GAPDH expression was used as an internal control. Each value indicates the mean ± SEM and is representative of results obtained from three independent experiments. versus control group; , versus LPS-stimulated group. DEX: dexamethasone.