Dietary TU-100 Alters Fecal Microbiota in a Dose-Dependent Manner. PCoA plots of unweighted UniFrac distance and weighted UniFrac distances were generated for fecal microbiotas at weeks 0 (a), 12 (b), and 24 (c). Red squares, blue triangles, orange circles, green triangles, and purple triangles indicate groups 1–5, respectively. Groups 1, 3, and 5 were fed AIN-76A and 0.75% and 3.0% TU-100 for 24 weeks, respectively. Groups 2 and 4 were fed 0.75% and 3.0% TU-100 for 12 weeks and then switched to AIN-76A for another 12 weeks, respectively. (a) At week 0, the fecal microbiotas in each group appeared to be randomly distributed. (b) At week 12, the plots of group 1, groups 2 and 3, and groups 4 and 5 appeared to be clustered, respectively, in PCoAs of unweighted UniFrac distances. (c) At week 24, the plots of groups 3, 4, and 5 appeared to be clustered. Group 2 was separated from group 3 and seemed to show similar distribution to group 1 in PCoAs of both unweighted and weighted UniFrac distances.