Manilkara zapota (L.) P. Royen Leaf Water Extract Induces Apoptosis in Human Hepatocellular Carcinoma (HepG2) Cells via ERK1/2/Akt1/JNK1 Signaling Pathways

Figure 8

Determination on the involvement of ROS in Manilkara zapota leaf water extract in HepG2 cells. HepG2 cells were cotreated with 50 μM α-tocopherol or ascorbic acid for 72 h. Values are reported as mean ± SD (n = 3). Value with different superscript letter indicates significant difference among the concentrations by Tukey’s test (P < 0.05). At 96 μg/mL, the cotreatment with 50 μM antioxidant α-tocopherol and Manilkara zapota leaf water extract significantly increased the viability of the cells to 57% (P < 0.05). At 48 μg/mL of Manilkara zapota leaf water extract, cotreatment with α-tocopherol significantly increased the viability of cells from 25% to 40% (P < 0.05).