Evidence-Based Complementary and Alternative Medicine

Research Article

Manilkara zapota (L.) P. Royen Leaf Water Extract Induces Apoptosis in Human Hepatocellular Carcinoma (HepG2) Cells via ERK1/2/Akt1/JNK1 Signaling Pathways

Table 1

Nucleotide sequence of PCR primers for amplification and sequence-specific detection of cDNA (obtained from the GenBank database).
Primer name
Oligonucleotides (5′-3′) sequence
ERK1/2  F– CCACCCATATCTGGAGCAGT  
R– CAGTCCTCTGAGCCCTTGTC
Akt1  F– AGAAGCAGGAGGAGGAGGAG  
R– TCTCCTTCACCAGGATCACC
JNK1  F– GTGATCAATGGCTCTCAGCA  
R– TGACTAACCGACTCCCCATC
iNOS  F– GTGGTGACAAGCACATTTGG  
R– GTCATGAGCAAAGGCACAGA
VEGFA  F– CCCACTGAGGAGTCCAACAT  
R– AAATGCTTTCTCCGCTCTGA
ACTF– AGAGCTACGAGCTGCCTGAC  
R– AGCACTGTGTTGGCGTACAG
GAPDF– GGATTTGGTCGTATTGGGC  
R– TGGAAGATGGTGATGGGATT
18S rRNF– GTAACCCGTTGAACCCCATT  
R– CCATCCAATCGGTAGTAGCG
ACTB: beta-actin; ERK1/2: extracellular signal-regulated kinase 1/2; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; iNOS: inducible nitric oxide synthase; JNK1: c-Jun N-terminal kinase 1; VEGFA: vascular endothelial growth factor A.
gene