Antipsoriatic Effects of Wannachawee Recipe on Imiquimod-Induced Psoriasis-Like Dermatitis in BALB/c Mice

Figure 4

Histological examinations of the six different treatment groups, stained with hematoxylin and eosin (H&E). (a) H&E stained dorsal skin and right ear pinna of control and IMQ-treated mice. Images magnified 100x (A, C, E, and G) and 200x (B, D, F, and H). (A, B) Control dorsal skin tissue section treated with Vaseline showing normal epidermis, dermis, sebaceous glands, and hair follicles. (C, D) Dorsal skin tissue section of IMQ-treated mice showing flaky crust, acanthosis, and hyperkeratosis of the epidermis. Abundant inflammatory infiltration and elongated rete ridges were also shown with IMQ treatment. (E, F) Control group ear pinna section showing normal epidermis, dermis, and cartilage. (G, H) Right ear pinna of IMQ-treated mice showing epidermal hyperplasia and disturbance in the normal pattern of the dermal layer. (b) Histopathology of acitretin- and WCR-treated dorsal skin sections (A, C, E, and G) and right ear pinna sections (B, D, F, and H) of IMQ-induced psoriasis-like dermatitis mice in different treatment groups. (A–H) After treatment for 10 days. (A, B) sections of IMQ-induced psoriasis-like dermatitis dorsal skin and right ear pinna treated with acitretin showed greatly decreased inflammatory infiltration and hyperplasia of the epidermis. (C, D) WCR 800 mg/kg treated mice showed slightly reduced inflammatory infiltrates relative to IMQ-treated mice. (E, F) WCR 1,600 mg/kg treated group had significant decreases in thickened epidermis, hyperplasia, and inflammatory cell infiltration. (G, H) WCR 3,200 mg/kg treated group showed maximum efficacy of treatment with recovered tissue having normal epidermis, dermis, sebaceous glands, and hair follicles. (c) H&E stained tissue sections from the spleens of mice in different treatment groups. (A) A sectional view of a control group spleen showing normal white pulp and normal red pulp. (B) A sectional view of an IMQ-treated mouse spleen showing depletion of white pulp. (C–F) Sectional views of acitretin, WCR 800, 1,600, and 3,200 mg/kg treated mice, respectively, showing dose-dependent depletion of white pulp. Ctrl, control; IMQ, imiquimod; Gr, group; DS, dorsal skin; and EP, ear pinna.

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