Figure 3: Effect of galangin and pinocembrin on hexokinase (HK) and pyruvate kinase (PK) activity in insulin-resistant HepG2 cells. Note: (a) galangin; (b) pinocembrin. HepG2 cells were plated in 96-well plates overnight, followed by treated in the absence or presence of 5x10-6mol/L insulin. After 36 h, cells were exposed to different concentrations of galangin and pinocembrin for 24h. Then the activity of intracellular hexokinase and pyruvate kinase was tested by hexokinase and pyruvate kinase assay kits. Values are means ± SEM from six separate determinations. Values with different letters (A-C, a–c) in the same column are significantly different from each other (p < 0.05).