Immunostimulatory Effect of Zanthoxylum schinifolium-Based Complex Oil Prepared by Supercritical Fluid Extraction in Splenocytes and Cyclophosphamide-Induced Immunosuppressed Rats

Figure 4

Effect of ZPCO on the concentration of cytokines in splenocytes. Cells were seeded into 96-well plates, followed by treatment with ZPCO (0, 5, 10, 30, 50, 100, or 300 μg/ml) or lipopolysaccharide (LPS; 10 μg/ml). Next, the cells were incubated for 24 h in a 5% CO₂ incubator, after which the levels of TNF-α, IFN-γ, IL-2, and IL-12 secretion into the culture medium were analyzed. Bars labeled with different superscripts have significantly different values (P < 0.05 versus control). Data are presented as means ± standard errors (n = 3).

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