Immunostimulatory Effect of Zanthoxylum schinifolium-Based Complex Oil Prepared by Supercritical Fluid Extraction in Splenocytes and Cyclophosphamide-Induced Immunosuppressed Rats

Figure 7

Effect of ZPCO on immunity-associated spleen damage in Sprague Dawley (SD) rats. SD rats were oral administrated with saline, cyclophosphamide (CY; 5 mg/kg/day), and ZPCO (0, 30, 100, or 300 mg/kg/day) once daily for 28 days, after which spleen damage was analyzed histologically. Representative images of the sectioned spleens of (a) normal rats (saline treatment), (b) control rats (treated with only CY), and (c–e) ZPCO-treated rats [treated with CY and (c) 30 mg/kg, (d) 100 mg/kg, or (e) 300 mg/kg ZPCO]. Scale bar = 200 μm. CV, central vein; LN, lymph nodule; MZ, marginal zone; RP, red pulp.