Involvement of Flavonoids from the Leaves of Carya cathayensis Sarg. in Sirtuin 1 Expression in HUVEC Senescence

Figure 4

Effect of SIRT1 on HUVECs senescence regulated by TFs. Panels (a and b): representative images of WB analysis and the semiquantification of SIRT1, E2F1, p53, and p21 in normal HUVECs. Panels (c and d): representative images of SA-β-gal staining and quantification of SA-β-gal positive cells (blue staining for the senescent cell). Scale bar = 100 μm. Panels (e–i): representative images of WB analysis and the semiquantification of SIRT1, p53, p21, and E2F1 in HUVECs. Values in panel are expressed as mean ± SD (n = 3). statistically significant difference compared to control. and versus control. ^# and ^## versus Ang II. ^■ and ^■■ versus TFs + Ang II.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)