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Evidence-Based Complementary and Alternative Medicine
Volume 2018, Article ID 8265150, 11 pages
Research Article

Small Peptides Compound Isolated from Agkistrodon with Antiarthritic Effect in Collagen-Induced Arthritis Rats

1Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China
2Zhejiang University of Technology, Hangzhou, Zhejiang 310014, China

Correspondence should be addressed to Li Xu; moc.361@bhhilux and Yong-Sheng Fan; moc.621@mctjzelppa

Received 30 November 2017; Revised 28 February 2018; Accepted 22 March 2018; Published 29 April 2018

Academic Editor: Antonella Fioravanti

Copyright © 2018 Lijun Mei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Agkistrodon in Chinese medicine has long been used as an effective treatment against rheumatoid arthritis (RA). The present research further investigated the effects of peptides extracted from the crude Agkistrodon on the RA rat model. Extracted peptides were separated by parameter-optimized ion-exchange chromatography (IEC), peptide fractions were further analysed by MALDI-TOF/TOF MS, and nano-LC-MS/MS acquired mass spectra were further characterized using Mascot software, which ranks the best matches in the NCBI database. RT-PCR results in RAW264.7 cells indicated that Agkistrodon peptide components had inhibitory effects against inflammatory cytokines. The therapeutic efficacy of Agkistrodon peptides was evaluated on the Wistar rats with collagen-induced arthritis. Symptom relief and reduced cartilage destruction and bone erosion were observed, which can be explained by the direct suppression of inflammatory cytokines in the joints. Agkistrodon peptides downregulate the expression of TNF-α, IL-1β, and IL-6, which may alleviate cartilage destruction and bone erosion, thus relieving symptoms of RA.