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Evidence-Based Complementary and Alternative Medicine
Volume 2018, Article ID 8298151, 9 pages
https://doi.org/10.1155/2018/8298151
Research Article

Antibacterial Effects of Leaf Extract of Nandina domestica and the Underlined Mechanism

1Lab of Medical Microbiology and Parasitology, Experimental Teaching Center of Basic Medicine, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China
2Department of Medical Laboratory, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200030, China
3Department of Immunology and Medical Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China

Correspondence should be addressed to Wei Zhao; moc.361@hstaiewoahz

Received 15 June 2017; Accepted 18 December 2017; Published 18 January 2018

Academic Editor: Laura De Martino

Copyright © 2018 Zhao-Yu Guo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim. The study was conducted to investigate the antibacterial and antiasthmatic effects of Nandina domestica leaf extract, to find out its active components, and to assess its safety issue. Methods. (1) Solid-phase agar dilution method was used for antibacterial activity test of nandina leaf extract and the change of bacterial morphology after treatment was observed under the transmission microscope; (2) guinea pig model of asthma was used to test the asthma prevention effect of nandina leaf extract; (3) alkaloids and flavones were separated from nandina leaf extract and were further analyzed with HPLC-MS; (4) mice model was used to assessment of the safety issue of nandina leaf extract. Results. (1) Nandina leaf extract inhibited the growth of bacteria and destroyed bacterial membrane; (2) nandina leaf extract alleviated animal allergy and asthma; (3) the components reextracted by ethyl acetate were active, in which alkaloids inhibited Gram-positive bacteria and prevented asthma and flavones inhibited Gram-negative bacteria; (4) nandina leaf extract had no toxic effect on mice. Conclusion. Nandina leaves inhibit bacterial growth and prevent asthma through alkaloids and flavones, which had integrated function against chronic bronchitis. This study provided theoretical basement for producing new Chinese medicine against chronic bronchitis.