Gynostemma pentaphyllum Attenuates the Progression of Nonalcoholic Fatty Liver Disease in Mice: A Biomedical Investigation Integrated with In Silico Assay
Gypenoside XL regulated PPARα expression and downstream target genes related to liver lipid metabolism. In NAFLD mice, the haptic PPARα protein level was significantly decreased after alcohol consumption. For SOX9 and BRD4 protein, the expression level was significantly increased. Gypenoside XL 10 and 20 mg/kg/day treatment led to a significant increase in PPARα protein level compared with model group (). However, the expression of SOX9 and BRD4 protein was not affected by GP treatment (a). CDAA diet and CCl4 exposure can lead to downregulation of ACO and CPT-1 mRNA level as well as protein level in mice liver. Gypenoside XL 10 and 20 mg/kg/day treatment can upregulate mRNA and protein levels of ACO and CPT-1 in NAFLD mice ((b), (c)). , compared with normal group and , compared with model group.