Bu Shen Yang Xue Prescription Has Treating Effect on Endometrial Cancer through FSH/PI3K/AKT/Gankyrin/HIF-α/cyclinD 1 Pathway in Ishikawa Cells

Figure 3

Effect of BSYX on cell ability and apoptosis rate in human endometrial carcinoma Ishikawa cells. Cells were treated with FSH, LY284002, blank serum and drug serum containing BSYX for 24 h, then cell ability was detected by CCK8 assay and was calculated (a). Cells were double stained with annexin V-FITC and PI and analyzed by flow cytometry. And percentage of apoptotic cells was shown by histogram ((b) and (c)). Data are expressed as the mean ±SEM n = 3 in each group. P<0.05,P<0.01 versus the control group.