JYYS Granule Mitigates Renal Injury in Clinic and in Spontaneously Hypertensive Rats by Inhibiting NF-B Signaling-Mediated Microinflammation

Figure 4

JYYS granule decreases protein levels of renal profibrogenic genes and extracellular matrix decomposition in SHR. ((a) and (b)) Immunofluorescence (IF) was carried out to examine the effects of JYYS granule on renal TGF-β1, collagen-IV, MMP-9, and TIMP-1 expression; and the semiquantification of these genes was based on the fluorescence intensity. Data are expressed as mean ± SD, n=10, #P <0.05 versus WKY group, Δ<0.05 versus SHR group. ((c) and (d)) Immunohistochemistry (IHC) was carried out to examine the effects of JYYS granule on renal TGF-β1, collagen-IV, MMP-9, and TIMP-1 expression and the semiquantification of these genes was based on the intensity. Data are expressed as mean ± SD, n=10, #P <0.05 versus WKY group, Δ<0.05 versus SHR group. (e) Western blot was carried out to examine the effects of JYYS granule on renal TGF-β1, collagen-IV, MMP-9, and TIMP-1 expression.