Anti-Inflammatory Effect of Gamisoyo-San in an Animal Model of Amyotrophic Lateral Sclerosis

<div><b>Effect of Gamisoyo-San (GSS) on oxidative stress in the muscle of </b><svg height="12.0705pt" id="M25" style="vertical-align:-0.2325001pt" version="1.1" viewbox="-0.0498162 -11.838 56.0471 12.0705" width="56.0471pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M442 495C434 557 427 602 423 639C405 645 348 665 284 665C142 665 55 579 55 469C55 373 127 319 229 267C304 227 348 190 348 131C348 67 309 25 248 25C152 25 93 138 73 202L38 190C45 130 58 46 68 21C81 15 100 7 128 -1C158 -10 193 -15 229 -15C375 -15 472 74 472 188C472 287 400 343 295 394C207 439 171 470 171 525C171 583 212 624 268 624C349 624 387 550 407 487L442 495Z" id="g13-81"></path></g><g transform="matrix(.013,0,0,-0.013,6.565,0)"><path d="M394 664C165 664 42 495 42 323C42 132 181 -15 381 -15C570 -15 725 116 725 332C725 529 576 664 394 664ZM374 622C491 622 571 503 571 304C571 120 494 27 399 27C271 27 196 171 196 343C196 516 274 622 374 622Z" id="g13-77"></path></g><g transform="matrix(.013,0,0,-0.013,16.353,0)"><path d="M41 650V615C111 608 121 602 121 516V134C121 48 111 42 32 35V0H289C413 0 518 27 592 83C668 141 717 229 717 351C717 446 678 517 628 564C564 625 467 650 341 650H41ZM255 540C255 569 257 586 265 595C272 602 292 609 328 609C397 609 450 589 491 547C537 502 566 426 566 331C566 152 474 43 340 43C270 43 255 68 255 140V540Z" id="g13-66"></path></g><g transform="matrix(.013,0,0,-0.013,26.246,0)"><path d="M409 0V34C329 39 322 56 322 125V635C234 611 160 593 94 583V554L145 551C186 547 190 541 190 488V125C190 54 181 39 102 34V0H409Z" id="g13-47"></path></g><g transform="matrix(.0091,0,0,-0.0091,32.746,-5.741)"><path d="M711 314H408V273C513 264 518 257 518 187V100C518 69 508 53 494 44C481 35 459 32 440 32C276 32 201 186 201 335C201 514 307 618 425 618C515 618 588 575 615 460L659 468C651 550 646 602 643 636C606 645 528 665 446 665C231 665 45 545 45 318C45 118 194 -15 431 -15C512 -15 600 7 661 22C656 48 655 80 655 116V191C655 260 661 263 711 273V314Z" id="g22-69"></path></g><g transform="matrix(.0091,0,0,-0.0091,39.462,-5.741)"><path d="M257 635C101 635 25 509 25 419C25 312 99 229 220 229C237 229 254 234 278 246L328 272C302 155 197 53 48 27L55 -15C99 -14 188 2 228 18C369 73 478 199 478 382C478 518 403 635 257 635ZM236 589C313 589 339 482 339 386C339 366 338 342 334 315C320 309 304 303 279 303C206 303 159 375 159 459C159 522 183 589 236 589Z" id="g22-55"></path></g><g transform="matrix(.0091,0,0,-0.0091,44.076,-5.741)"><path d="M310 382C345 404 366 417 384 433C404 450 417 474 417 501C417 578 357 635 252 635H251C184 635 135 603 107 572L57 506L86 478C122 527 156 554 205 554C250 554 290 528 290 467S227 367 144 340L151 300C168 304 190 308 208 308C260 308 327 275 327 181C327 89 288 49 238 49C177 49 131 93 109 111C95 122 82 119 70 110C57 100 40 82 40 63C40 48 48 34 65 21C86 4 126 -12 177 -12C240 -12 460 60 460 222C460 298 409 363 310 380V382Z" id="g22-49"></path></g><g transform="matrix(.0091,0,0,-0.0091,48.724,-5.741)"><path d="M698 0V42C632 50 620 61 587 147C521 317 460 491 396 665L338 652L135 141C103 59 91 50 23 42V0H252V42C181 50 179 63 192 108C204 146 216 179 225 206H418C432 170 446 131 456 102C471 60 465 50 409 42V0H698ZM398 265H244C270 336 294 407 321 478H324L398 265Z" id="g22-63"></path></g></svg><b> mice</b>. GSS reduces oxidative stress-related proteins in the gastrocnemius (GA) muscle of <svg height="12.0836pt" id="M26" style="vertical-align:-0.2455997pt" version="1.1" viewbox="-0.0498162 -11.838 54.2071 12.0836" width="54.2071pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M409 504C401 567 396 607 392 642C354 654 312 665 266 665C137 665 60 583 60 487C60 374 161 325 225 290C300 250 355 215 355 141C355 68 311 21 235 21C131 21 86 122 71 183L41 176C48 128 61 42 68 21C78 16 93 8 118 0C142 -7 175 -15 216 -15C349 -15 438 69 438 174C438 287 344 333 265 374C186 414 138 449 138 522C138 576 172 631 249 631C336 631 363 562 380 499L409 504Z" id="g121-81"></path></g><g transform="matrix(.013,0,0,-0.013,6.162,0)"><path d="M381 665C170 665 44 498 44 318C44 125 188 -15 369 -15C552 -15 703 117 703 333C703 534 550 665 381 665ZM359 629C491 629 601 517 601 306C601 114 502 21 390 21C249 21 146 158 146 346S248 629 359 629Z" id="g121-77"></path></g><g transform="matrix(.013,0,0,-0.013,15.756,0)"><path d="M43 650V622C120 616 128 612 128 526V124C128 39 120 33 34 27V0H270C392 0 492 25 567 83C643 141 690 230 690 350C690 444 655 517 605 565C543 625 450 650 323 650H43ZM213 547C213 587 217 598 226 604C236 612 262 617 304 617C371 617 429 604 474 576C554 529 592 439 592 336C592 176 505 36 319 36C246 36 213 55 213 131V547Z" id="g121-66"></path></g><g transform="matrix(.013,0,0,-0.013,25.311,0)"><path d="M384 0V27C293 34 287 42 287 114V635C232 613 172 594 109 583V559L157 557C201 555 205 550 205 499V114C205 42 199 34 109 27V0H384Z" id="g113-50"></path></g><g transform="matrix(.0091,0,0,-0.0091,31.551,-5.741)"><path d="M700 305H441V272C542 264 548 258 548 186V107C548 64 536 53 516 42C493 30 462 24 429 24C237 24 151 189 151 333C151 517 264 626 415 626C507 626 585 594 609 470L642 476C634 544 629 601 626 636C594 644 513 665 429 665C234 665 46 554 46 321C46 123 194 -15 415 -15C495 -15 578 7 642 22C635 50 635 81 635 116V201C635 259 639 264 700 272V305Z" id="g58-69"></path></g><g transform="matrix(.0091,0,0,-0.0091,38.148,-5.741)"><path d="M247 635C116 635 39 518 39 421C39 315 113 237 221 237C239 237 258 241 281 253L349 289C315 140 204 42 60 19L66 -15C90 -15 153 -5 207 16C344 70 447 199 447 385C447 521 373 635 247 635ZM231 598C329 598 356 478 356 390C356 371 355 347 352 325C330 309 297 297 262 297C179 297 127 369 127 456C127 515 156 598 231 598Z" id="g50-58"></path></g><g transform="matrix(.0091,0,0,-0.0091,42.581,-5.741)"><path d="M290 377C321 398 342 415 358 430C378 450 389 473 389 502C389 578 329 635 238 635H237C184 635 137 610 109 578L64 515L88 493C112 529 154 573 208 573S303 542 303 482C303 409 233 370 141 341L149 308C165 313 190 319 215 319C272 319 341 283 341 193C342 98 292 43 222 43C163 43 122 72 96 94C88 101 79 100 70 94C61 87 47 73 46 60C44 47 48 37 62 23C76 10 118 -12 165 -12C238 -12 430 62 430 223C430 297 379 359 290 375V377Z" id="g50-52"></path></g><g transform="matrix(.0091,0,0,-0.0091,47.045,-5.741)"><path d="M682 0V33C615 38 603 49 571 134C507 305 441 494 378 665L343 655L136 136C101 48 86 41 22 33V0H246V33C170 41 161 50 183 112C196 152 209 192 224 232H441C461 176 478 126 493 87C504 49 499 41 433 33V0H682ZM425 279H242C273 363 303 448 335 533H337L425 279Z" id="g58-63"></path></g></svg> mice. Representative images show immunoblots for (a) heme oxygenase 1 (HO1) or (b) ferritin in lysates from the GA muscle of mice in non-Tg, Tg, and GSS-treated Tg groups (n= 4/group). Data represent the means ± SEM. Statistical significance was calculated by ANOVA, <svg height="6.01072pt" id="M27" style="vertical-align:-0.04980993pt" version="1.1" viewbox="-0.0498162 -5.96091 7.75925 6.01072" width="7.75925pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M471 153C471 170 463 194 452 212C400 220 373 229 322 255C373 281 400 290 452 298C463 316 471 339 471 357C456 366 431 371 410 370C377 329 356 310 308 279C311 336 317 364 336 413C326 432 310 451 294 459C279 451 262 432 252 413C271 364 277 336 280 279C232 310 211 329 178 370C157 371 132 367 117 357C117 340 125 316 136 298C188 290 215 281 266 255C215 229 188 220 136 212C125 194 117 171 117 153C132 144 157 139 178 140C211 181 232 200 280 231C277 174 271 146 252 97C262 78 278 59 294 51C309 59 326 78 336 97C317 146 311 174 308 231C356 200 377 181 410 140C431 139 456 143 471 153Z" id="g113-43"></path></g></svg>p&lt;0.05, <svg height="6.01072pt" id="M28" style="vertical-align:-0.04980993pt" version="1.1" viewbox="-0.0498162 -5.96091 15.4189 6.01072" width="15.4189pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M471 153C471 170 463 194 452 212C400 220 373 229 322 255C373 281 400 290 452 298C463 316 471 339 471 357C456 366 431 371 410 370C377 329 356 310 308 279C311 336 317 364 336 413C326 432 310 451 294 459C279 451 262 432 252 413C271 364 277 336 280 279C232 310 211 329 178 370C157 371 132 367 117 357C117 340 125 316 136 298C188 290 215 281 266 255C215 229 188 220 136 212C125 194 117 171 117 153C132 144 157 139 178 140C211 181 232 200 280 231C277 174 271 146 252 97C262 78 278 59 294 51C309 59 326 78 336 97C317 146 311 174 308 231C356 200 377 181 410 140C431 139 456 143 471 153Z" id="g113-43"></path></g><g transform="matrix(.013,0,0,-0.013,7.631,0)"><path d="M471 153C471 170 463 194 452 212C400 220 373 229 322 255C373 281 400 290 452 298C463 316 471 339 471 357C456 366 431 371 410 370C377 329 356 310 308 279C311 336 317 364 336 413C326 432 310 451 294 459C279 451 262 432 252 413C271 364 277 336 280 279C232 310 211 329 178 370C157 371 132 367 117 357C117 340 125 316 136 298C188 290 215 281 266 255C215 229 188 220 136 212C125 194 117 171 117 153C132 144 157 139 178 140C211 181 232 200 280 231C277 174 271 146 252 97C262 78 278 59 294 51C309 59 326 78 336 97C317 146 311 174 308 231C356 200 377 181 410 140C431 139 456 143 471 153Z" id="g113-43"></path></g></svg>p&lt;0.01, <svg height="6.01072pt" id="M29" style="vertical-align:-0.04980993pt" version="1.1" viewbox="-0.0498162 -5.96091 7.75925 6.01072" width="7.75925pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M471 153C471 170 463 194 452 212C400 220 373 229 322 255C373 281 400 290 452 298C463 316 471 339 471 357C456 366 431 371 410 370C377 329 356 310 308 279C311 336 317 364 336 413C326 432 310 451 294 459C279 451 262 432 252 413C271 364 277 336 280 279C232 310 211 329 178 370C157 371 132 367 117 357C117 340 125 316 136 298C188 290 215 281 266 255C215 229 188 220 136 212C125 194 117 171 117 153C132 144 157 139 178 140C211 181 232 200 280 231C277 174 271 146 252 97C262 78 278 59 294 51C309 59 326 78 336 97C317 146 311 174 308 231C356 200 377 181 410 140C431 139 456 143 471 153Z" id="g113-43"></path></g></svg> <svg height="6.01072pt" id="M30" style="vertical-align:-0.04980993pt" version="1.1" viewbox="-0.0498162 -5.96091 7.75925 6.01072" width="7.75925pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M471 153C471 170 463 194 452 212C400 220 373 229 322 255C373 281 400 290 452 298C463 316 471 339 471 357C456 366 431 371 410 370C377 329 356 310 308 279C311 336 317 364 336 413C326 432 310 451 294 459C279 451 262 432 252 413C271 364 277 336 280 279C232 310 211 329 178 370C157 371 132 367 117 357C117 340 125 316 136 298C188 290 215 281 266 255C215 229 188 220 136 212C125 194 117 171 117 153C132 144 157 139 178 140C211 181 232 200 280 231C277 174 271 146 252 97C262 78 278 59 294 51C309 59 326 78 336 97C317 146 311 174 308 231C356 200 377 181 410 140C431 139 456 143 471 153Z" id="g113-43"></path></g></svg><svg height="6.01072pt" id="M31" style="vertical-align:-0.04980993pt" version="1.1" viewbox="-0.0498162 -5.96091 7.75925 6.01072" width="7.75925pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M471 153C471 170 463 194 452 212C400 220 373 229 322 255C373 281 400 290 452 298C463 316 471 339 471 357C456 366 431 371 410 370C377 329 356 310 308 279C311 336 317 364 336 413C326 432 310 451 294 459C279 451 262 432 252 413C271 364 277 336 280 279C232 310 211 329 178 370C157 371 132 367 117 357C117 340 125 316 136 298C188 290 215 281 266 255C215 229 188 220 136 212C125 194 117 171 117 153C132 144 157 139 178 140C211 181 232 200 280 231C277 174 271 146 252 97C262 78 278 59 294 51C309 59 326 78 336 97C317 146 311 174 308 231C356 200 377 181 410 140C431 139 456 143 471 153Z" id="g113-43"></path></g></svg>p&lt;0.001.</div>

Evidence-Based Complementary and Alternative Medicine

fig2

Figure 2

Figure 2: Anti-Inflammatory Effect of Gamisoyo-San in an Animal Model of Amyotrophic Lateral Sclerosis 