Sipjeondaebo-tang Alleviates Oxidative Stress-Mediated Liver Injury through Activation of the CaMKK2-AMPK Signaling Pathway

Figure 1

The effect of Sipjeondaebo-tang (SDT) on AA plus iron-mediated apoptosis. (a) UPLC chromatogram of nine marker compounds in SDT. The chromatograms were obtained at 280 nm (for 5-hydroxymethyl-2-furfural, calycosin-7-O-β-D-glucoside, cinnamic acid, and 6-gingerol), 230 nm (for paeoniflorin and ferulic acid), 203 nm (for ginsenoside Rg1), 254 nm (for glycyrrhizic acid), and 330 nm (for decursin), respectively. (b) Cell viability. HepG2 cells were pretreated with 30-1000 μg/mL of SDT for 1 h, exposed to 10 μM of AA for 12 h, and treated with 5 μM iron for 1 h. Relative cell viabilities were measured by MTT assay. (c) Changes on apoptosis-related proteins. Cells were incubated with 300 μg/mL of SDT for 1 h and then treated with AA plus iron, as described in panel (b). Equal protein loading was verified by β-actin immunoblotting (left). The intensities of cleaved forms of PARP and caspase-3 were quantified by scanning densitometry (middle and right). All values represent mean ± SD of three separated experiments; significant versus untreated control, ^∗∗P < 0.01; significant versus AA plus iron, ^##P < 0.01 and ^#P < 0.05.

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