Sipjeondaebo-tang Alleviates Oxidative Stress-Mediated Liver Injury through Activation of the CaMKK2-AMPK Signaling Pathway

Figure 3

The effect of SDT on AMPK activation. (a) AMPK phosphorylation. Immunoblot analyses were conducted using HepG2 cell lysates that had been treated with SDT (300 μg/mL) for indicated time periods. Equal protein loading was verified by β-actin immunoblotting (left). Relative levels of phosphorylated AMPK (middle) and phosphorylated ACC (right) were quantified by scanning densitometry. (b) Effect of compound C on SDT-mediated mitochondrial protection. 10 μM of compound C-pretreated HepG2 cells (1 h) were exposed to SDT, AA, and iron. (c) Effect of compound C on SDT-mediated cytoprotection. Expression of procaspase 3 (left) and relative cell viability (right) were monitored by immunoblot and MTT analysis, respectively. All values represent mean ± SD of three separated experiments; significant versus untreated control, ^∗∗P < 0.01 and ^∗P < 0.05; significant versus AA plus iron, ^##P < 0.01 and ^#P < 0.05; NS, not significant.

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