A Chinese Herbal Preparation, Xiao-Er-An-Shen Decoction, Exerts Neuron Protection by Modulation of Differentiation and Antioxidant Activity in Cultured PC12 Cells

Figure 1

The representative HPLC-MS chromatogram of XEASD. (a) Structures of chemical markers identified in XEASD extract, including liquiritin (1), calycosin 7-O-β-glucoside (2), ammonium glycyrrhizinate (3), naringin (4), 3,6′-disinapoylsucrose (5), hesperidin (6), neohesperidin (7), astragaloside IV (8), and puerarin (internal standard, ISTD). (b) The representative LC-MS chromatogram of XEASD extract. The LC condition was indicated in the Materials and Methods. The denotations from 1 to 8 in the chromatogram correspond to the chemical markers as shown in (a). The identification of the chemical markers was analyzed by a MS detector in a negative mode except astragaloside IV (8) in a positive mode. Representative chromatograms were shown, .

(a)

(b)