Biological Evaluation of Different Extracts of Aerial Parts of Nepeta deflersiana and Standardization of Active Extracts Using 8-Epi-7-Deoxyloganic Acid and Ursolic Acid by Validated HPTLC Method

Figure 6

Quantification of 8-epi-7-deoxyloganic acid 1 and Ursolic acid 2 in different fractions of N. deflersiana by HPTLC using chloroform, methanol, and formic acid (8.9:0.8:0.3, v/v/v) as mobile phase. (a) Chromatogram of standard 1 ( = 0.07; 800 ng/spot) and 2 ( = 0.57; 800 ng/spot) at λmax= 515 nm. (b) Pictogram of p-anisaldehyde derivatized TLC plate at day light. (c) N. deflersiana n-butanol extract [NDBE (1, spot 2, = 0.07; 2, spot 8, = 0.57)]; (b) N. deflersiana ethyl acetate extract [NDEE (1, spot 2, = 0.07; 2, spot 8, = 0.57)].

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