Total Flavone of Rhododendron Improves Cerebral Ischemia Injury by Activating Vascular TRPV4 to Induce Endothelium-Derived Hyperpolarizing Factor-Mediated Responses

Figure 7

Laser scanning confocal analysis of Ca²⁺ fluorescence intensity in rat cerebral basilar artery smooth muscle cells. In each group the drugs/chemicals were injected via tail vein 30 min before ischemia, and all rats were killed after ischemia for 25 min followed by 2 h of reperfusion. (A) Cells pretreated with Fluo-3/AM. (a) Ca²⁺ fluorescence intensity in Sham group; (b) Ca²⁺ fluorescence intensity in Ischemic group; (c) Ca²⁺ fluorescence intensity in TFR group; (d) Ca²⁺ fluorescence intensity in TFR+HC-067047 group; (e) Ca²⁺ fluorescence intensity in TFR+Apamin group; (f) Ca²⁺ fluorescence intensity in TFR+TRAM-34 group. (B) Effect of TFR and each channel blocker on Ca²⁺ fluorescence intensity of cerebral basilar artery smooth muscle cells in rats of ischemia/reperfusion injury. ^∗P <0.01 versus Sham; ^#P<0.05, ^##P<0.01 versus Model (Ischemic); ^▲▲P<0.01 versus TFR.