Research Article

UHPLC-ESI-MS Analysis of Purified Flavonoids Fraction from Stem of Dendrobium denneaum Paxt. and Its Preliminary Study in Inducing Apoptosis of HepG2 Cells

Figure 4

Effect of fraction II on the morphological changes in HepG2 cells using fluorescence microscopy. Nuclear staining with Hoechst 33258 using fluorescence microscopy showed that fraction II induces typical cell apoptosis including membrane blebbing, cytoplasm shrinkage, nuclear chromatin condensation, and nucleoli fragmentation.