UHPLC-ESI-MS Analysis of Purified Flavonoids Fraction from Stem of Dendrobium denneaum Paxt. and Its Preliminary Study in Inducing Apoptosis of HepG2 Cells

Table 1

MS date for characterization of compounds in Dendrobium denneaum paxt. by UHPLC-ESI-MS/MS.

Peak number	RT (min)	RRT	Negative ions ()	MS^n	Identification
(3)	9.5	0.24	593 [M-H]^–	MS^2: 503.19, 473.01 (100), 383.11, 353.09	Apigenin-6,8-di-c-β-D-glucoside
(4)	11.98	0.28	563 [M-H]^–	MS^2: 503.08, 473.04 (100), 443.08, 383.10, 353.07	Apigenin-6-C-β-D-xyloside-8-C-β-D-glucoside
(5)	14.53	0.26	563 [M-H]^–	MS^2: 503.07, 473.03 (100), 443.10, 383.06, 353.05	Isoschaftoside
(6)	15.2	0.17	563 [M-H]^–	MS^2: 473.07, 443.10 (100), 383.10, 353.09	Schaftoside
(7)	17.58	0.09	771 [M-H]^–	MS^2: 609.22 (100), 462.98; MS^3: 301.06	Quercetin-3-O-rutinoside-7-O-glucoside
(8)	19.57	0.26	563 [M-H]^–	MS^2: 473.09, 443.10 (100), 383.18, 353.12	Apigenin-6-C-β-D-glucoside-8-C-β-D-xyloside
(9)	21.6	1	563 [M-H]^–	MS^2: 413.04 (100), 293.06; MS^3: 293.08	Apigenin-6-C-glucosyl-2′′-O-xyloside
(10)	24.05	1.09	563 [M-H]^–	MS^2: 413.04 (100), 293.10; MS^3: 293.09	Apigenin-8-C-glucosyl-2′′-O-xyloside
(11)	25.95	0.5	609 [M-H]^–	MS^2: 300.02 (100), 271.06 MS^3: 271.03, 255.05, 179.00, 150.94	Quercetin-7-O-rutinoside
(12)	28.05	2.02	609 [M-H]^–	MS^2: 301.04 (100), 271.09; MS^3: 271.07, 255.08, 179.00, 151.01	Rutin
(13)	33.13	0.13	593 [M-H]^–	MS^2: 285.0; MS^3: 267.06, 257.06 (100), 225.16, 229.06, 212.99	Kaempferol-3-O-rutinoside
(14)	38.86	0.39	593 [M-H]^–	MS^2: 285.07; MS^3: 267.19, 257.13 (100), 229.01, 213.18	Kaempferol-7-O-rutinoside
(15)	42.05	0.13	577 [M-H]^–	MS^2: 269.18 (100), 225.07	Apigenin-7-O-rutinoside