Subchronic and Genetic Safety Assessment of a New Medicinal Dendrobium Species: Dendrobium Taiseed Tosnobile in Rats

Table 7

Revertant changes of DTTE on Salmonella typhimurium strains mutagenicity test in the presence and absence of S9 mixture.

Compound	Conc. (mg/plate)	S9	Number of revertants (colony/plate)
			TA98	TA100	TA102	TA1535	TA1537
Negative control	0	−
DTTE	0.3125	−
	0.625	−
	1.25	−
	2.5	−
	5.0	−
4-NQO		−
NaN3		−
MMC		−
9-AA		−
Negative control		+
DTTE	0.3125	+
	0.625	+
	1.25	+
	2.5	+
	5.0	+
2-AA		+

Data was presented as mean ± SD ( replicate plates); negative control for Salmonella strains was added with sterile reversed osmosis water; positive reagents without S-9 mix reactions were 2.5 μg/plate 4-nitroquinoline-N-oxide (4-NQO) for TA98, 5 μg/plate sodium azide (NaN₃) for TA100, TA1535, and 0.5 μg/plate mitomycin C (MMC) for TA102 and 50 μg/plate 9-aminoacridine (9-AA) for TA1537; positive reagent with S-9 mix reactions was 5 μg/plate 2-aminoanthracene (2-AA) for all tester strains. difference of colonies more than twofold of negative control and treated groups at .