Bioactivity-Guided Isolation of Neuritogenic Factor from the Seeds of the Gac Plant (Momordica cochinchinensis)

Figure 2

Separations: extraction method 1 (chemical): chemical extractions of MCS seeds were carried out using absolute ethanol, ether, hot ethanol, and ethyl acetate. Solvents were evaporated, reconstituted in ethanol and dilutions prepared in HBSS. Fractionation Schematic Solvent/Protein Extraction Method 2 (protein): Plant Total Protein Extraction Kit PE0230 (Sigma-Aldrich, St. Louis, MO) was used for isolation, and all washes were kept for analysis. All washings as well as the protein isolate were evaporated and diluted in HBSS and evaluated for neuritogenic activity in PC-12 cells. All chemical fractions, methanol, and acetone washes failed to produce neuritogenic effects.