Bioactivity-Guided Isolation of Neuritogenic Factor from the Seeds of the Gac Plant (Momordica cochinchinensis)

Figure 3

Gel excision layout: total seed proteins (native [A] and reduced) with β-Me [B] were separated using a gradient SDS PAGE gel 4–20% Mini-PROTEAN® TGX™ at 200V for 45 minutes. Gels were stained with Blue-band it®, washed in ultrapure water, then excised and electroeluted back into solution at 200V, reconstituted in HBSS, and evaluated for biological neuritogenic activity on PC-12 cells. All gel sections by process of procedural elimination left only two small biologically active (nonvisual) bands at around 16-17kDa (C45D) containing the predominant neuritogenic active fraction.