6-Gingerol Activates PI3K/Akt and Inhibits Apoptosis to Attenuate Myocardial Ischemia/Reperfusion Injury
Pretreatment with 6-G (6 mg/kg) inhibited myocardial apoptosis induced by I/R. (a) Representative images from the TUNEL assays (×200) in the myocardial tissues. Green fluorescence represents TUNEL-positive nuclei; blue fluorescence represents nuclei of total cardiac myocytes. (b) Apoptosis index. (c) Caspase-3 expression in myocardial homogenates was evaluated using ELISA. (d) Expression level of cleaved caspase-3 in the myocardial tissues detected using western blot and quantitative analyses (e). Note that against the Sham group; against the I/R group.