Evidence-Based Complementary and Alternative Medicine

Research Article

Investigating the Antiproliferative and Antioxidant Properties of Pancratium maritimum L. (Amaryllidaceae) Stems, Flowers, Bulbs, and Fruits Extracts

Table 3

Antioxidant activity of P. maritimum extracts.


P. maritimum	DPPH test (IC₅₀ μg/mL)	ABTS test (IC₅₀ μg/mL)	β-carotene bleaching test (IC₅₀ μg/mL)	β-carotene bleaching test (IC₅₀ μg/mL)	FRAP test μM Fe (II)/g	RACI	GAS

			30 min	60 min

Flowers

ETP	32.2 ± 3.2	16.7 ± 1.6	88.7 ± 8.1	72.5 ± 7.1	3.8 ± 0.4	0.05	1.93
ET1	81.3 ± 8.1	10.4 ± 1.2	9.2 ± 0.9	19.4 ± 2.0	15.6 ± 1.2	-0.22	1.18
AQ	49.7 ± 4.7	14.4 ± 1.4	8.2 ± 8.1	7.2 ± 1.5	NA	-0.31	0.24
ET2	95.3 ± 9.1	11.6 ± 1.1	63.6 ± 6.3	64.1 ± 6.2	10.1 ± 1.0	0.15	2.26

Fruits

ETP	35%	13.3 ± 1.3	48.2%	37.7%	NA	-0.38	0.02
ET1	846.1 ± 44.2	6.9 ± 0.6	63.3 ± 6.1	77.5 ± 7.1	11.8 ± 1.1	0.56	2.96
AQ	40.4%	98.2 ± 9.6	11.2 ± 1.3	51.1 ± 5.1	2.4 ± 0.3	0.04	1.95
ET2	746.8 ± 48.2	10.0 ± 1.0	94.9 ± 9.2	80.9 ± 8.1	1.8 ± 0.1	-0.16	0.92

Stems

ETP	711.5 ± 23.5	23.4 ± 2.4	57.8 ± 5.9	89.1 ± 8.7	5.1 ± 0.5	0.54	2.60
ET1	84.1 ± 8.4	8.1 ± 0.8	81.6 ± 8.2	98.0 ± 9.4	19.7 ± 1.4	0.23	2.94
AQ	38.5%	110.9 ± 15.3	11.4 ± 1.2	40.5 ± 4.0	NA	-0.09	1.10
ET2	105.7 ± 15.2	16.4 ± 1.4	95.1 ± 9.1	81.6 ± 8.2	5.6 ± 0.6	-0.33	0.19

Bulbs

ETP	923.0 ± 50.6	272.4 ± 16.5	84.2 ± 8.1	13.2 ± 1.1	NA	0.24	3.01
ET1	46%	8.9 ± 0.9	50.2 ± 48.2	9.9 ± 0.9	NA	-0.32	0.63
AQ	149.3 ± 14.2	231.8 ± 22.6	21.5 ± 2.3	23.9 ± 2.4	4.2 ± 0.3	-0.15	1.69
ET2	42.2%	12.0 ± 1.1	9.4 ± 8.7	8.0 ± 7.1	NA	0.52	3.50

Positive control

Ascorbic acid	5.0 ± 0.8	1.7 ± 0.06
Propyl gallate			0.09 ± 0.004	0.09 ± 0.004
BHT					63.2 ± 4.3

Data are expressed as mean ± standard deviation (SD) (n= 3). ETP: petroleum ether extract; ET1: sequential extraction with ethanol; AQ: sequential extraction with water; ET2: maceration with ethanol; RACI: Relative Antioxidant Capacity Index; GAS: Global Antioxidant Score. DPPH Radical Scavenging Activity Assay: one-way ANOVA <0.0001 followed by multicomparison Dunnett’s test: <0.01 compared with ascorbic acid. ABTS test: one-way ANOVA <0.0001 followed by a multicomparison Dunnett’s test: <0.01 compared with ascorbic acid. -carotene bleaching test at 30 minutes of incubation: one-way ANOVA <0.0001 followed by multicomparison Dunnett’s test: <0.01 compared with propyl gallate. -carotene bleaching test at 60 minutes of incubation: one-way ANOVA <0.0001 followed by multicomparison Dunnett’s test: <0.01 compared with propyl gallate. Ferric Reducing Antioxidant Power (FRAP): one-way ANOVA <0.0001 followed by multicomparison Dunnett’s test <0.01 compared with BHT.