Modified Si-Ni-San Decoction Ameliorates Central Fatigue by Improving Mitochondrial Biogenesis in the Rat Hippocampus

Table 3

Selected separation condition of HPLC. The sample of MSNS was analysed by HPLC; the selected separation condition was presented in the table.

Mobile phase	Gradient condition	Flow rate	Capillary temperature	Injection volume	Detection wavelength
A: phosphate buffer (PH=2)	0~5min: 5%~5%B;	0.8ml/min	35°C	10μL	222nm
	5~30min:5%~15%B;
B: acetonitrile	30~60min:15%~25%B;
	60~70min:25%~40%B;
	70~80min:40%~95%B;
	80~85min:95%~95%B