Modified Si-Ni-San Decoction Ameliorates Central Fatigue by Improving Mitochondrial Biogenesis in the Rat Hippocampus

Table 4

Selected separation condition of UPLC-ESI-MS. The sample of MSNS was analysed by UPLC-ESI-MS; the selected separation condition was presented in the table.

Mobile phase	Gradient condition	Flow rate	Capillary temperature	Injection volume	Detection wavelength
A: 0.05%formic acid-water	0~4.5min: 5%~5%B;	0.3ml/min	35°C	2.6μL	222nm
	4.5~10.42min:5%%~13%B;
	10.42~21.42min:13%%~13%B;
	21.42~53.42 min,13%%~29%B;
B: acetonitrile	53.42~59.87 min,29%%~66%B;
	59.87~63.87 min,66%%~95%B;
	63.87~73 min,95%%~95%B