Inhibition of α-Glucosidase, Intestinal Glucose Absorption, and Antidiabetic Properties by Caralluma europaea

Figure 3

Kinetics of α-glucosidase inhibition by ethyl acetate fraction (EACe). (a) Release rate of glucose depending on the concentration of sucrose. (b) Lineweaver-Burk plot of kinetic analysis of α-glucosidase inhibition by EACe. α-glucosidase was treated with various concentrations of sucrose (1-2-3-4-6 mM) in the absence or presence of EACe at two different doses (500 and 1000 μg/mL). The enzymatic reaction was performed by incubating the mixture at 37°C for 20 minutes. Glucose released was detected colorimetrically by adding a commercial test kit.

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