Evidence-Based Complementary and Alternative Medicine / 2018 / Article / Fig 2

Research Article

Aster glehni Extract Containing Caffeoylquinic Compounds Protects Human Keratinocytes through the TRPV4-PPARδ-AMPK Pathway

Figure 2

Western blot analyses for PPAR, AMPK, and SPTLC2 in HaCaT cells treated with Aster glehni extract, SDS, DNCB, and GSK0660. AG increased the protein levels of biomarkers related to the protection of keratinocytes in DNCB (a) or SDS (b) treated condition. At large, the effects of AG were offset by PPARδ antagonist treatment. The results are expressed as means ± SEM. N=3 for each group. Values were statistically analyzed by unpaired t-test and one-way ANOVA. All experiments were repeated over three times. The dash-dotted line means the one-way ANOVA result between ctrl and all other groups. The dotted line means the one-way ANOVA result among all groups except for control. Meaning of indications: Ctrl is an untreated control group, DNCB is a DNCB treated group, D+AG is a DNCB+AG treated group, D+A+GSK is a DNCB+AG+GSK0660 treated group, SDS is a SDS-treated group, S+AG is a SDS+AG treated group, and S+A+GSK is a SDS+AG+GSK0660 treated group.
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