Prevention of Prostate Tumor Development by Stimulation of Antitumor Immunity Using a Standardized Herbal Extract (Deep Immune®) in TRAMP Mice

Figure 3

The DI stimulates nitric oxide (NO) production by upregulation of NOS2 expression in macrophages. Protein was extracted from cultured RAW264.7 macrophages after 24 h of stimulation with 100 μg/mL of herbal extract () or medium only (Control, ). (a) Western blot analysis of NOS2 protein expression. (b) NO production in RAW264.7 macrophage cultures stimulated with different concentrations of herbal extract (0, 25, 50, or 100 μg/mL) for 24 h. The data were presented as mean ± SD of each treatment ( compared to “0” control, one-way ANOVA, ). NO production (over 10 μM) in LPS-stimulated cultures was not included.

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