Acmella oleracea (L) R. K. Jansen Reproductive Toxicity in Zebrafish: An In Vivo and In Silico Assessment

Figure 7

Representative pictures of zebrafish embryos and larvae from groups (a), (b), (c), and (d), whose parental generation was exposed to EHFAo at 50, 100, and 200 μg/L. (a) Normal embryo with two cells (0.75 hpf); (b) normal embryo with 16 cells (1.5 hpf); normal embryo (c); and larva (f) at 24 and 48 hpf. White arrows indicate tail malformation in (e), (g), (h), (l), (m), and (n). Cardiac edema in 24 hpf embryo is shown in (d), and 96 hpf larvae in (i), (h), and (k) (red arrows). In (n) a fish with retarded growth is observed (white dashed arrow).