Evaluation of Alternative Methods to Assess the Biological Properties of Propolis on Metabolic Activity and Biofilm Formation in Streptococcus mutans
Metabolic activity in S. mutans biofilm measured by flow cytometry. The biofilm cellular suspension was excited at 488 nm, and emission was registered with FITC channel for Syto 9 (530/30) and PerCP channel (670/LP) for propidium iodide. (a) Untreated cells. (b) Chlorhexidine. (c) EP at 0.8 μg·mL−1. (d) EP at 0.4 μg·mL−1. (e) EP at 0.2 μg·mL−1. (f) EP at 0.1 μg·mL−1. EP, polyphenol-rich extract of propolis.