Citrus Alkaline Extract Delayed the Progression of Pulmonary Fibrosis by Inhibiting p38/NF-B Signaling Pathway-Induced Cell Apoptosis

Figure 5

Effects of CAE on the expression of related marker proteins in pulmonary tissue of BLM-induced pulmonary fibrosis mice. (a) The expressions of Pro-SPC in the lung tissues were measured by immunohistochemical assay (Magnification, 200x). (b) The expressions of vimentin in the lung tissues were determined by immune-fluorescent methods (Magnification, 200x). (c) The quantitative result of Pro-SPC. (d) The quantitative result of vimentin. Normal: normal group, water; BLM: model group, BLM+water; L: CAE-16 group, BLM+ CAE at a dose of 16mg/kg once per day; M: CAE-32 group, BLM+ CAE at a dose of 32mg/kg once per day; H: CAE-64 group, BLM+ CAE at a dose of 64mg/kg once per day; Prednisone: prednisone group, BLM+ prednisone at a dose of 6mg/kg once per day; Pirfenidone: pirfenidone group, BLM+ prednisone at a dose of 100mg/kg once per day. All data are expressed as mean ±SD (n=6), versus normal group; versus BLM group.

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