Citrus Alkaline Extract Delayed the Progression of Pulmonary Fibrosis by Inhibiting p38/NF-B Signaling Pathway-Induced Cell Apoptosis

Figure 6

Effects of CAE on the cell apoptosis in pulmonary tissue of BLM-induced pulmonary fibrosis mice. (a) The apoptotic cells in pulmonary tissue were stained by the Tunel test kit and observed with a fluorescence microscope. (Magnification, 200x). (b) The quantitative result of TUNEL staining. Normal: normal group, water; BLM: model group, BLM+water; L: CAE-16 group, BLM+ CAE at a dose of 16mg/kg once per day; M: CAE-32 group, BLM+ CAE at a dose of 32mg/kg once per day; H: CAE-64 group, BLM+ CAE at a dose of 64mg/kg once per day; Prednisone: prednisone group, BLM+ prednisone at a dose of 6mg/kg once per day; Pirfenidone: pirfenidone group, BLM+ prednisone at a dose of 100mg/kg once per day. All data are expressed as mean ±SD (n=6), versus normal group; versus BLM group.

(a)

(b)