Piper cubeba L. Methanol Extract Has Anti-Inflammatory Activity Targeting Src/Syk via NF-B Inhibition

Figure 2

Effect of Pc-ME on proinflammatory cytokines and NF-B transcription factor. (a) Semiquantitative PCR for iNOS and IL-6 expression in RAW264.7 cells (2 x 10⁶ cells/ml)induced by LPS for 6 h with 50 and 100 μg/ml of Pc-ME. (b) HEK 293 cells (2 x 10⁵ cells/ml) were cotransfected with NF-κB-Luc, β-gal (0.8 μg), and MyD88 or TRIF for 48 h with or without 50 and 100 μg/ml of Pc-ME. (c) Immunoblot for p65 and p50 in the nuclear fraction of LPS-treated RAW264.7 cells (5 x 10⁶ cells/ml) with or without 100 μg/ml of Pc-ME. p < 0.05 and p < 0.01 compared to control groups. All data (b) are expressed as mean ± SD 3 replicates.

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