Piper cubeba L. Methanol Extract Has Anti-Inflammatory Activity Targeting Src/Syk via NF-B Inhibition

Figure 3

Pc-ME anti-inflammatory effects on NF-B signaling. (a) RAW264.7 cells (2 x 10⁶ cells/ml) were treated with LPS for various times with or without 100 μg/ml of Pc-ME. Phosphorylated and total IκBα, IKKα/β, AKT, p85, Syk, and Src were evaluated by immunoblot. (b) Syk and Src were transfected into HEK293 cells (2 x 10⁵ cells/ml) for overexpression with 50 and 100 μg/ml of Pc-ME. Syk and Src phosphorylation were evaluated. (c) NO production with Syk and Src inhibitors, 20 μM of piceatannol and PP2, respectively, in LPS-induced RAW264.7 cells (10⁶ cells/ml) within 24 h. p < 0.05 compared to control or normal groups. All data (c) are expressed as mean ± SD of 3 replicates. WCL: whole cell lysates.

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