Combined Extracts of Herba Epimedii and Fructus Ligustri Lucidi Rebalance Bone Remodeling in Ovariectomized Rats

Figure 5

Effects of HE & FLL on RANKL, M-CSF, ATP6v0d2, and Wnt5a in OVX rats. (a) The mRNA expressions of RANKL, M-CSF, ATP6v0d2, and Wnt5a were measured by qRT-PCR analysis with β-actin as an internal control. (b) The protein expressions of RANKL, M-CSF, ATP6v0d2, and Wnt5a were measured by immunofluorescence staining. (c) Immunofluorescence images of RANKL, M-CSF, ATP6v0d2, and Wnt5a. Data are represented as mean ± SEM, n = 6. Note: < 0.01 versus Sham; < 0.05 and < 0.01 versus OVX.

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