Eucommia ulmoides Oliv. Leaf Extract Improves Erectile Dysfunction in Streptozotocin-Induced Diabetic Rats by Protecting Endothelial Function and Ameliorating Hypothalamic-Pituitary-Gonadal Axis Function

Figure 5

EULE restored eNOS and nNOS protein expression in diabetic rats. (a) Western blot analysis demonstrated the effect of EULE on the expression of nNOS, phosphor-eNOS, and eNOS protein in diabetic rats. (b, d) Relative expression levels of nNOS and eNOS normalized to β-actin expression. (c) Phosphorylated eNOS levels normalized to total eNOS levels. (e) The mRNA expression levels of iNOS was analyzed by real-time PCR and expressed as percentages of Actb levels. The data are presented as the mean ± SEM. p < 0.05 versus the control group; # p < 0.05, ## p < 0.01, and ### p < 0.001 versus the DM group (n = 10 in each group).

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