Protective Effect of the Total Triterpenes of Euscaphis konishii Hayata Pericarp on Bacillus Calmette-Guérin Plus Lipopolysaccharide-Induced Liver Injury
Figure 4
TTEP alleviated inflammation in BCG/LPS-induced liver injury. (a) An equal amount (50 μg) of total liver homogenate proteins from each group was detected with β-actin as a control. Western blot analysis results show the effect of TTEP on the protein levels of TLR4, NF-κB p65, Phospho-NF-κB p65, IκB-β, and Phospho-IκB-β. (b) The nuclear and cytosolic fraction of liver homogenate were separated, and then detected by 8-15% SDS-PAGE. The protein expressions of nuclear NF-κB p65 was detected using Histone as the control, while the cytosolic NF-κB p65 levels were measured with β-actin as the control. (c) Total RNA of liver tissue extracted with TRIzol reagent and then transcribed reversely into cDNA. Quantitative real-time PCR analysis results show the effect of TTEP on the mRNA levels of TLR4, NF-κB, iNOS, TNF-α, and IL-6 of each group. Results are presented as means ± SD of three independent experiments, , compared with the model group; ##P<0.01 compared with the control group.