Protective Effect of the Total Triterpenes of Euscaphis konishii Hayata Pericarp on Bacillus Calmette-Guérin Plus Lipopolysaccharide-Induced Liver Injury

Table 1

List of primers in PCR amplification.

Gene	Accession No.	Primer Sequence	Amplicon Size (bp)
TLR4	NM_021297.3	Forward: 5'-TAGCCATTGCTGCCAACATC-3' Reverse: 5'- CCTCAGCAGGGACTTCTCAA -3'	194
NF-κB	AY521463.1	Forward: 5'- GGAGACATCCTTCCGCAAAC -3' Reverse: 5'- AGGTCCTTCCTGCCCATAAC -3'	103
iNOS	M87039.1	Forward: 5'- ATGCGAAAGGTCATGGCTTC-3' Reverse: 5'- CCCAAATGTGCTTGTCACCA -3'	198
TNF-α	D84199.2	Forward: 5'- CCCAAAGGGATGAGGTGAGT-3' Reverse: 5'- GGTCTGGGCCATAGAACTGA -3'	185
IL-6	M24221.1	Forward: 5'- ACTTCACAAGTCCGGAGAGG-3' Reverse: 5'-TGCAAGTGCATCATCGTTGT -3'	174
Nrf2	BC026943	Forward: 5'-TGGCTGATACTACCGCTGTT-3' Reverse: 5'-TGGAGAGGATGCTGCTGAAA-3'	162
HO-1	NM_010442.2	Forward:5'-TGTCTGAGGCCTTGAAGGAG-3' Reverse: 5'-CAGGGCCGTGTAGATATGGT-3'	146
NQO1	BC004579	Forward: 5'-ACAGGTGAGCTGAAGGACTC-3' Reverse: 5'-CCAAACCACTGCAATGGGAA-3'	155
SOD1	NM_011434.2	Forward: 5'-TCCACGTCCATCAGTATGGG-3' Reverse: 5'- AATGGACACATTGGCCACAC -3'	164
β-actin	M12481.1	Forward: 5'-CCAGCCTTCCTTCTTGGGTA-3' Reverse: 5'-CAATGCCTGGGTACATGGTG-3'	143

Full-length gene sequences were obtained from the Nucleotide database (www.ncbi.nlm.nih.gov/nuccore/), and primers were designed using primer premier 5.0 software.