Granule of BU-XIN RUAN-MAI Attenuates the Patients’ Angina Pectoris of Coronary Heart Disease via Regulating miR-542-3p/GABARAP Signaling

Figure 3

GABARAP is required for the inhibitory activity of Granule of BU-XIN RUAN-MAI on oxidation and inflammation in vivo and in vivo. (a, b) Granule of BU-XIN RUAN-MAI increased the GABARAP protein expression in heart tissues of ISO-induced rats; versus the sham group and versus the model group. (c) Granule of BU-XIN RUAN-MAI increased the GABARAP mRNA expression in heart tissues of ISO-induced rats after administration with Granule of BU-XIN RUAN-MAI for 6 days; versus the sham group and versus the model group. (d) Granule of BU-XIN RUAN-MAI increased the GABARAP mRNA expression in angiotensin II-induced HUVEC; versus the sham group and versus the placebo group. (e) Real-time PCR analysis of the GABARAP expression in rat heart and HUVEC cells after knockdown of GABARAP for 48 h; versus the AAV-vector group or vector plasmid. (f) Real-time PCR analysis of PPARα expression in rat heart after injection of adeno-associated virus followed by administration with Granule of BU-XIN RUAN-MAI for 6 days; versus the sham + placebo + AAV-vector and versus the ISO + placebo + AAV-vector. (g) Real-time PCR analysis of PPARα expression in HUVEC after transfection for 48 h followed by Granule of BU-XIN RUAN-MAI incubation for 24 h; versus the sham + placebo + vector, and versus the angiotensin II + placebo + vector. (h) Real-time PCR analysis of IL-6 expression in rat heart after injection of adeno-associated virus followed by administration with Granule of BU-XIN RUAN-MAI for 6 days; versus the sham + placebo + AAV-vector and versus the ISO + placebo + AAV-vector. (i) Real-time PCR analysis of IL-6 expression in HUVEC after transfection for 48 h followed by Granule of BU-XIN RUAN-MAI incubation for 24 h; versus the sham + placebo + vector and versus the angiotensin II + placebo + vector. (j) Real-time PCR analysis of IL-1β expression in rat heart after injection of adeno-associated virus followed by administration with Granule of BU-XIN RUAN-MAI for 6 days; versus the sham + placebo + AAV-vector and versus the ISO + placebo + AAV-vector. (k) Real-time PCR analysis of IL-1β expression in HUVEC after transfection for 48 h followed by Granule of BU-XIN RUAN-MAI incubation for 24 h; versus the sham + placebo + vector and versus the angiotensin II + placebo + vector. (l) Real-time PCR analysis of TNFα expression in rat heart after injection of adeno-associated virus followed by administration with Granule of BU-XIN RUAN-MAI for 6 days; versus the sham + placebo + AAV-vector and versus the ISO + placebo + AAV-vector. (m) Real-time PCR analysis of TNFα expression in HUVEC after transfection for 48 h followed by Granule of BU-XIN RUAN-MAI incubation for 24 h; versus the sham + placebo + vector and versus the angiotensin II + placebo + vector. (n) Real-time PCR analysis of SOD1 expression in rat heart after injection of adeno-associated virus followed by administration with Granule of BU-XIN RUAN-MAI for 6 days; versus the sham + placebo + AAV-vector and versus the ISO + placebo + AAV-vector. (o) Real-time PCR analysis of SOD1 expression in HUVEC after transfection for 48 h followed by Granule of BU-XIN RUAN-MAI incubation for 24 h; versus the sham + placebo + vector and versus the angiotensin II + placebo + vector. The data are representative of three independent experiments.

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