Granule of BU-XIN RUAN-MAI Attenuates the Patients’ Angina Pectoris of Coronary Heart Disease via Regulating miR-542-3p/GABARAP Signaling

Figure 4

miR-542-3p is involved in Granule of BU-XIN RUAN-MAI-induced upregulation of GABARAP expression. (a) Real-time PCR of GABARAP expression in HUVEC cells and the cells were incubated with both ActD and Granule of BU-XIN RUAN-MAI for 10 h; versus the DMSO + placebo and versus DMSO + Granule of BU-XIN RUAN-MAI. (b) Predication of binding site of miR-542-3p on GABARAP 3′UTR by Targetscan (http://www.targetscan.org/vert_72/). (c) Real-time PCR analysis of miR-542-3p in rat heart after Granule of BU-XIN RUAN-MAI administration as described above; versus the sham and versus ISO + placebo. (d) Real-time PCR analysis of miR-542-3p in HUVEC after Granule of BU-XIN RUAN-MAI incubation; versus the sham and versus angiotensin II + placebo. (e) Real-time PCR analysis of GABARAP in HUVEC and H9C2 cells after transfection for 48 h followed by Granule of BU-XIN RUAN-MAI incubation for 24 h; versus placebo + Mock and versus placebo + miR-542-3p. (f) Real-time PCR analysis of GABARAP in HUVEC and H9C2 cells after transfection for 48 h; versus Mock. (g) Luciferase reporter assay analysis of pmir-GABARAP-3′UTR luciferase activity after transfection for 48 h; versus Mock. (h) Real-time PCR analysis of GABARAP expression after transfection for 48 h, versus Mock. (i) Western blot analysis of GABARAP expression after transfection for 48 h. The data are representative of three independent experiments.

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