Potential of Regular Consumption of Cameroonian Neem (Azadirachta indica L.) Oil for Prevention of the 7,12-Dimethylbenz(a)anthracene-Induced Breast Cancer in High-Fat/Sucrose-Fed Wistar Rats

Figure 5

Effects of neem (Azadirachta indica) oil on microphotographs H&E 400× of mammary glands (a), uterine (b), vagina (c), liver (d), and kidneys (e) after 12 weeks of treatment. NOR-1: normal rats fed with SD treated with cotton oil; NOR-2: normal rats fed with HFHS treated with cotton oil; DMBA: negative control treated with cotton oil; TAM + DMBA: animals treated with tamoxifen (3.3 mg/kg); Neem + DMBA: animals treated with neem (Azadirachta indica) oil at a dose of 3 mL/kg during three different frequencies. All groups except the normal groups (NOR-1 and NOR-2) received subcutaneous dose of DMBA at the dose of 50 mg/kg. Data are represented as mean ± SEM (n =5). La: lumen of alveoli; At: adipose tissue; Se: eosinophil secretion, L: lobular; HLU: hyperplastic lobular unit; St: stroma, En: endometrium, Lu: lumen of uterine; Vp: portal vein, H: hepatocyte, S: sinusoids; Dt: distal tube, G: glomerulus; Ge: stratum germinativum, Gr: stratum granulosum, C: stratum corneum.