Potential of Regular Consumption of Cameroonian Neem (Azadirachta indica L.) Oil for Prevention of the 7,12-Dimethylbenz(a)anthracene-Induced Breast Cancer in High-Fat/Sucrose-Fed Wistar Rats

Table 2

Effects of neem (Azadirachta indica) oil on some tumor parameters of rats treated for 12 weeks.

Items	NOR	DMBA	Tamox + DMBA	Neem 1 + DMBA	Neem 2 + DMBA
Number of rats with tumors/total rats	0/5	5/5	3/5	5/5	4/5
Tumor incidence (%)	0	100 ^###	60	100	80
Average tumors weight (mg/kg)	-	545.34±43.11	203.26±14.21	355.25±16.07	348.84±53.42
% inhibition related to tumor weight	-	-	62.73	34.86	36.03
Total tumor burden (g)	0	3.27	1.21	2.10	2.09
% inhibition related to tumor burden	-	-	62.99	35.78	36.09
Tumor volume (Cm^3)	-	701.01± 104.25	289.96±118.91	223.09± 62.52	139.77±30.83

NOR-1: normal rats fed with SD treated with cotton oil; NOR-2: normal rats fed with HFHS treated with cotton oil; DMBA: negative control treated with cotton oil; TAM + DMBA: animals treated with tamoxifen (3.3 mg/kg); Neem + DMBA: animals treated with neem (Azadirachta indica) oil at a dose of 3 mL/kg at three different frequencies. All groups except the normal groups (NOR-1 and NOR-2) received subcutaneous dose of DMBA at the dose of 50 mg/kg. Data are represented as mean ± SEM (n = 5). ###p < 0.001 as compared to normal rats (NOR-2); p < 0.05, p < 0.01, p < 0.001 as compared to DMBA.