Potential of Regular Consumption of Cameroonian Neem (Azadirachta indica L.) Oil for Prevention of the 7,12-Dimethylbenz(a)anthracene-Induced Breast Cancer in High-Fat/Sucrose-Fed Wistar Rats
Table 3
Effects of neem (Azadirachta indica) oil on organs weight of rats treated for 12 weeks.
Organs
NOR
DMBA
Tamox + DMBA
Neem 1 + DMBA
Neem 2 + DMBA
Estrogen target organs
Uterus
1469.48± 102.53
1582.36± 93.59
819.54± 77.55
1577.11± 158.96
1981.88± 141.37
Ovaries
723.19± 72.11
882.51± 44.50
409.33± 14.03
683.52± 32.22
836.13± 16.01
Endometrium height
14.58± 0.22
13.30± 2.72
7.52± 1.75
5.76± 0.21
4.60± 0.01
Vaginal height
24.34± 0.83
20.34± 0.50
18.34± 0.03
13.51± 0.52
8.51± 1.02
Other organs
Liver
18544.58± 550.16
17195.25± 462.30
15956.35±230.36
18047.65± 478.10
20497.02± 313.70
Lungs
5507.55±275.62
4296.46± 170.08
4078.32±133.02
4377.30± 258.86
5988.14± 223.65
Spleen
1606.99± 57.93
1221.45± 52.61#
1442.81± 76.46
1450.50± 91.55
1432.21± 68.33
Adrenals
392.50± 32.89
620.63± 22.76##
510.04± 23.65
453.95± 62.17
314.61± 13.52
Kidneys
3508.11± 90.54
3574.44±81.16
3621.88± 65.18
3800.66± 175.32
3812.29±102.00
Femur
1676.57± 50.72
1581.92± 36.14
1399.61± 75.42
1523.45±105.61
1492.18± 42.24
Brain
5893.52± 75.05
6623.14± 121.70
6854.20± 223.93
7579.03± 122.88
6713.79± 342.75
NOR-1: normal rats fed with SD treated with cotton oil; NOR-2: normal rats fed with HFHS treated with cotton oil; DMBA: negative control treated with cotton oil; TAM + DMBA: animals treated with tamoxifen (3.3 mg/kg); Neem + DMBA: animals treated with neem (Azadirachta indica) oil at a dose of 3 mL/kg at three different frequencies. All groups except the normal groups (NOR-1 and NOR-2) received subcutaneous dose of DMBA at the dose of 50 mg/kg. Data are represented as mean ± SEM (n = 5). #p < 0.05, ##p < 0.01 as compared to normal rats (NOR-2); p < 0.05, p < 0.01, p < 0.001 as compared to DMBA.
