Potential of Regular Consumption of Cameroonian Neem (Azadirachta indica L.) Oil for Prevention of the 7,12-Dimethylbenz(a)anthracene-Induced Breast Cancer in High-Fat/Sucrose-Fed Wistar Rats
Table 4
Effects of neem (Azadirachta indica) oil on some biochemical and hematological parameters.
Items
NOR
DMBA
Tamox + DMBA
Neem 1 + DMBA
Neem 2 + DMBA
Transaminases
ALT
63.5± 2.37
69.33± 2.86
65.2± 2.35
55.16± 3.28
79.1± 1.21
AST
45.5± 0.79
47.33± 1.28
49.6± 4.91
42.5± 1.85
61.2± 0.71
Hematological parameter
WBC (×103μL−1)
13.3± 0.66
8.33± 0.54
10.77± 1.67
8.37± 1.67
5.5± 0.17
Lymphocytes (%)
43.2± 0.66
50.43± 1.43
50.775± 2.33
56.45± 1.69
53.6± 0.45
Monocytes (%)
18.95± 0.67
19.4± 1.52
18.875± 1.41
18.075± 1.13
18.3± 0.28
Granulocytes (%)
37.85±1.34
30.16± 0.94
24.225±1.53
25.475± 2.21
27.85±0.44
RBC (×103μL−1)
5.57± 0.16
1.40± 0.03###
1.21± 0.14
1.395 ± 0.07
1.07± 0.09
Hematocrit (%)
7.58± 0.66
7.36± 0.13
6.95± 0.93
6.55± 0.68
6.1± 0.28
MCV (fL)
173.7± 19.37
102.23± 2.42#
147.56± 9.98
121.15±6.79
145.45± 5.55
Platelets (×103μL−1)
1007± 116.62
2721.5± 28.86###
2419.75±72.64
1453.06± 84.64
1416.03± 10.69
MCH (pg)
7.5875± 0.66
7.36±0.13
6.95± 0.93
6.55± 0.68
6.1± 0.28
Hemoglobin (g/dL)
139.5± 6.20
143± 1.32
149± 3.31
137.25±3.00
154.75± 0.33
MCHC (g/dL)
2493± 222.73
1945± 35.79
3159.37± 122.81*
2460.33± 324.42
2374±67.70
NOR-1: normal rats fed with SD treated with cotton oil; NOR-2: normal rats fed with HFHS treated with cotton oil; DMBA: negative control treated with cotton oil; TAM + DMBA: animals treated with tamoxifen (3.3 mg/kg); Neem + DMBA: animals treated with neem (Azadirachta indica) oil at a dose of 3 mL/kg at three different frequencies. All groups except the normal groups (NOR-1 and NOR-2) received subcutaneous dose of DMBA at the dose of 50 mg/kg. Data are represented as mean ± SEM (n = 5). #p < 0.05, ###p < 0.001 as compared to normal rats (NOR-2); p < 0.05, p < 0.001 as compared to DMBA.
