Evidence-Based Complementary and Alternative Medicine

Research Article

Comparative Studies on Chemical Contents and Effect in Kidney-Yang Deficiency Rats of Salt-Processed Product and Wine-Processed Product of Cuscutae Semen

Table 1

Method validation of fifteen analytes in UPLC-MS quantitation.


Number	t_R (min)	Analytes	Formula	Regression equation	r²	Linear range (ng/ml)	LOD (μg/mL)	LOQ (μg/mL)	Precision (n = 6)	Repeatability (n = 6)	Stability (0–24 h)

1	5.51	3-CQA	C₁₆H₁₈O₉	y = 0.0775x − 2872.8378	0.9988	20341–244093	0.0014	0.0041	1.96	3.86	5.79
2	16.73	4-CQA	C₁₆H₁₈O₉	y = 0.1345x − 4699.9042	0.9991	3872–46461	0.0028	0.0084	0.33	0.44	0.57
3	1.46	3,4-DiCQA	C₂₅H₂₃O₁₂	y = 0.3181x − 65.9357	0.9990	206–2478	0.0112	0.0225	0.0019	0.0017	0.02
4	2.34	3,5-DiCQA	C₂₅H₂₃O₁₂	y = 0.2140x − 56.4874	0.9997	462–7394	0.4436	1.3309	0.0037	0.013	0.06
5	2.93	4,5-DiCQA	C₂₅H₂₃O₁₂	y = 0.1230x − 274.8306	0.9974	477–7636	0.4582	1.3745	0. 01	0.03	0.31
6	5.85	CA	C₉H₈O₄	y = 0.0003x − 0.4863	0.9915	5–75	0.0026	0.0105	0.03	0.07	0.16
7	7.73	5-FQA	C₁₀H₁₀O₄	y = 0.3482x − 18.5959	0.9998	206–2470	0.0001	0.0004	0.0019	0.0006	0.0072
8	4.04	p-Hydroxycinnamic acid	C₉H₈O₃	y = 0.1221x − 345.2182	0.9921	488–7806	0.0004	0.0014	0.03	0.03	0.37
9	10.03	Hyperoside	C₂₁H₂₀O₁₂	y = 0.0638x − 4194.4234	0.9991	6833–109333	0.0036	0.0109	—	—	—
10	13.29	Astragalin	C₂₁H₂₀O₁₁	y = 0.1698x − 10717.3813	0.9995	2683–42933	0.0172	0.0687	0.13	0. 4	0.09
11	23.75	Quercetin	C₁₅H₁₀O₇	y = 0.0035x − 69.1497	0.9989	202–2424	0.0006	0.0012	0.28	0.43	0.64
12	27.77	Kaempferol	C₁₅H₁₀O₆	y = 0.2544x − 406.6164	0.9974	5684–68210	0.0008	0.0017	0.24	0.09	0.63
13	28.54	Isorhamnetin	C₁₆H₁₂O₇	y = 0.0190x − 1032.1204	0.9967	1351–16210	8.76E − 05	0.0004	0.32	0.73	1.18
14	10.4	Isoquercitrin	C₂₁H₂₀O₁₂	y = 0.0473x − 2285.4629	0.9990	2567–41067	0.0020	0.0082	1.26	0.50	1.29
15	16.12	Luteolin-7-O-glucoside	C₂₁H₂₀O₁₁	y = 0.4190x − 2399.9345	0.9978	2196–26358	0.0004	0.0013	0.06	0.02	0.02

In the regression equation, x is the peak area, y the concentration of each analyte (ng/mL), and r the correlation coefficient. LOD, limit of detection (S/N = 3); LOQ, limit of quantification (S/N = 10). Precision, repeatability, and stability are shown in RSD (%).